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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and therefore might add to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a crucial position in selling cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two more splice variants had been reported but detailed nucleotide sequence data just isn't yet readily available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged via the antibody.
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This information outlines more feasible optimization of cellular and immunoassay parameters to guarantee the very best effects are...
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This guideline outlines even more achievable optimization of mobile and immunoassay parameters to guarantee the best possible results are...
Antibodies bound to the blocking peptide no more bind for the epitope on the focus on protein. By evaluating the staining from your blocked antibody vs . the antibody alone, one can see which staining is precise.
Target Information Cyclin E belongs for the really conserved cyclin household, whose associates are characterised by a spectacular periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit unique expression and degradation designs which lead to the temporal coordination of each and every mitotic party. Cyclin E forms a posh with and capabilities being a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates within the G1-S period boundary and it is degraded as cells development by S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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